Active Ingredient Profile

Bacillus subtilis Biofungicide

Biological Fungicide (Living Bacterium)

Bacillus subtilis is a naturally occurring soil bacterium used as a biological fungicide. It works by colonizing plant surfaces and outcompeting disease-causing fungi for space and nutrients. Also produces antifungal compounds (lipopeptides) that directly inhibit fungal growth. Organic approved.

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Classification
Biological Fungicide (Living Bacterium)
Signal Word
Exempt
Mode of Action
Competitive exclusion + antibiosis: colonizes plant surfaces, outcompetes fungi, produces antifungal lipopeptides

Target Pests

Powdery mildew, botrytis (gray mold), bacterial leaf spot, fire blight suppression, Rhizoctonia, Fusarium, Pythium, downy mildew, rust, Sclerotinia. Works best as a preventive - much less effective curatively. Best for vegetables, fruits, ornamentals, and greenhouse crops.

Products and Brand Names

Cease (BioWorks), Serenade Garden Disease Control (Bayer - most popular consumer brand), Rhapsody (BioWorks), Companion (Growth Products), Subtilex (Becker Underwood). Available as liquid concentrate and wettable powder.

๐Ÿ“‹ Safety Data Sheet (SDS)

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Bacillus subtilis Biofungicide โ€” Safety Data Sheet

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๐Ÿ“„ Bacillus subtilis Biofungicide โ€” Safety Data Sheet ยท View the complete SDS document above or download below

Safety and Precautions

Essentially non-toxic. OMRI listed for organic production. No REI (re-entry interval), no PHI (pre-harvest interval). Safe around children, pets, wildlife, and beneficial insects including pollinators. Can be applied up to and including the day of harvest.

Zero-day PHI: One of the biggest advantages of B. subtilis products - you can spray your tomatoes today and eat them tomorrow. No waiting period. This makes it ideal for home vegetable gardens.

Pro Tips

Preventive, not curative: B. subtilis must be present on the plant surface BEFORE the disease arrives. Think of it as a living shield. Once disease is established, it cannot cure it. Begin applications early and maintain regular coverage (every 7-14 days).

Compatibility: B. subtilis is a living organism. Do not tank-mix with fungicides that kill bacteria (copper, chlorothalonil). If using both, apply copper first, wait 5-7 days, then begin B. subtilis applications.

Best results: Apply in early morning or late afternoon to avoid UV degradation. The bacteria need time to colonize the plant surface before strong sunlight kills them. Adding a spreader-sticker improves adhesion and coverage.

Soil health bonus: When B. subtilis washes off leaves into the soil, it continues working as a beneficial soil microbe - suppressing root rot fungi (Pythium, Rhizoctonia, Fusarium) and promoting plant growth through nutrient cycling.

Did you know? Bacillus subtilis is one of the most studied bacteria in science - it was the first Gram-positive bacterium to have its entire genome sequenced (1997). It has been used in traditional Japanese fermented soybean food (natto) for over a thousand years.
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Reviewed by Derek GiordanoContent reviewed by a licensed pest management professional. Last reviewed: April 2026.
๐Ÿ“š Sources: EPA Pesticide Labels ยท NPIC Pesticide Info
Published: Jan 1, 2025 ยท Updated: Apr 7, 2026